More than 200 Virgin Trains ticket office staff are on strike in a row about plans to cut booking office window openings.
The 24-hour strike by members of the Transport Salaried Staff Association (TSSA) will affect stations across the country, including Euston in London, Coventry, Wolverhampton, Crewe, Preston and Glasgow.
The TSSA has accused Virgin Trains of closing windows to force passengers to use station ticket machines which offer fewer but more expensive tickets.
Gerry Doherty, general secretary of the TSSA, said: "This is all about defending a vital service to rail passengers who are already being ripped off with the most expensive fares in Europe."
But a spokesman for Virgin Trains told Personnel Today there had been "low take-up" of the strike.
He said: "The strike has had almost no impact whatsoever. There is only one station where we can't sell tickets over the counter and that's Lancaster."